Correlation of Toxicity with Lead Content in Root Tip Cells (Allium cepa L.) |
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Authors: | Ingrid Carruyo Yusmary Fernández Letty Marcano Xiomara Montiel Zaida Torrealba |
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Institution: | (1) Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela;(2) Urbanización Maracaibo, AV 12 A N° 66-66, Maracaibo, Estado Zulia, Venezuela |
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Abstract: | The present study determines lead content in onion root tip cells (Allium cepa L.), correlating it with its toxicity. The treatment was carried at 25 ± 0.5°C using aqueous solutions of lead chloride at
0.1, 0.25, 0.50, 0.75, and 1 ppm for 12, 24, 48, and 72 h. For each treatment, a control where the lead solution was substituted
by distilled water was included. After treatment, the meristems were fixed with a mixture of alcohol–acetic acid (3:1) and
colored according to the technique of Feulgen. Lead content was quantified by graphite furnace absorption atomic spectrometry.
The lead content in the roots ranged from 3.25 to 244.72 μg/g dry weight, with a direct relation with the concentration and
time of exposure. A significant negative correlation was presented (r = −0.3629; p < 0.01) among lead content and root growth increment, and a positive correlation (r = 0.7750; p < 0.01) with the induction of chromosomic aberrations. In conclusion, lead is able to induce a toxic effect in the exposed
roots, correlated with its content. |
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Keywords: | GFAAS Root cells Lead Toxicity |
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