A platelet factor stimulating human normal glial cells. |
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Authors: | B Westermark A Wasteson |
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Affiliation: | 1. The Wallenberg Laboratory, University of Uppsala, S-75122 Uppsala, Sweden;2. Institute of Medical and Physiological Chemistry, University of Uppsala, S-75122 Uppsala, Sweden |
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Abstract: | Multiplication stimulating activities of human serum and fractions thereof have been determined as stimulation of [3H]thymidine incorporation of serum-deprived human glial cells. Serum prepared from cell-free plasma had a considerably lower activity than serum prepared from whole blood. The major part of the growth-promoting activity of serum could be ascribed to a platelet factor, released during the coagulation process. The factor was trypsin-labile and heat-stable. A partial purification of the factor was achieved by ion exchange chromatography on CM-Sephadex at neutral pH. The purified material was 600–700 times as active as normal human serum on a protein basis. |
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