Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing |
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Authors: | Stølhaug Anne Bergh Kåre |
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Institution: | Department of Medical Microbiology, Laboratory Center, St. Olavs Hospital, N-7006 Trondheim, Norway. |
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Abstract: | Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established. |
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