Isolation and characterization of a heteropolysaccharide from the corm of Amorphophallus campanulatus |
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| Authors: | Debsankar Das Sadhan K Roy Bibhas Bhunia Syed S Islam |
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| Institution: | a Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India
b Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721 302, West Bengal, India |
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| Abstract: | A water-soluble polysaccharide isolated from the aqueous extract of the corm of Amorphophallus campanulatus was found to contain d-galactose, d-glucose, 4-O-acyl-d-methyl galacturonate, and l-arabinose in a molar ratio 2:1:1:1. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This molecule showed splenocyte activation. |
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| Keywords: | Amorphophallus campanulatus Corm polysaccharide Structure NMR spectroscopy Splenocyte activation |
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