Design of a thermostable cell adhesion protein |
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Authors: | Eiry Kobatake Koji Onoda Yasuko Yanagida Tetsuya Haruyama Masuo Aizawa |
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Institution: | (1) Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan |
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Abstract: | The design principle of a thermostable functional protein has been proposed by demonstrating genetic engineering synthesis of a thermostable cell adhesion protein. The cell adhesive peptide sequence, Arg-Gly-Asp (RGD), was incorporated into the elastin-based polyhexapeptide, whose repeating unit is Ala-Pro-Gly-Val-Gly-Val (APGVGV). The resulting protein possesses cell adhesion activity approximately 80% of fibronectin. After autoclaving at 120 °C for 20 min, the protein retained over 90% of cell adhesion activity, while the activity of autoclaved fibronectin decreased to 50%. |
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Keywords: | cell adhesion elastin polyhexapeptide RGD sequence thermostavle protein |
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