Isolation of complement protein D from urine of patients with Fanconi's syndrome |
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| Authors: | J E Volanakis K J Macon |
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| Institution: | 1. Department of Marine Ecosystems'' Functioning, Institute of Oceanography, University of Gdańsk, Al. Marszałka Piłsudskiego 46, Gdynia 81-378, Poland;2. Department of Pharmaceutical Biochemistry, Medical University of Gdańsk, Dębinki 1, Gdańsk 80-211, Poland;3. Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Al. Gen. J. Hallera 107, Gdańsk 80-416, Poland |
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| Abstract: | Complement protein D is the least abundant of all complement proteins and, thus, one of the most difficult to purify. We report a new method for obtaining pure D from urine of patients with Fanconi's syndrome. The method is simple and allows the purification of milligram amounts of D within a few days. It involves three chromatographic steps using Bio-Rex 70, hydroxylapatite HPLC, and reverse-phase HPLC. Protein D purified by this method is suitable for both functional and structural studies. |
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