| 大鼠星形胶质细胞组织因子活性的表达及其调控 |
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| 作者姓名： | Zhu FM Wen ZB He XF Li JC He SL |
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| 作者单位： | 1. 浙江省血液中心,杭州,310020
2. 湖南医科大学生理教研室,长沙,410078 |
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| 摘 要： | 本实验观察了基础培养条件和凝血酶刺激条件下,大鼠星形胶质细胞组织因子活性的表达及其信号传递途径。结果显示,基础条件下,Ａ２３１８７（４ｂｒｏｍｏｃａｌｃｉｕｍｉｏｎｏｐｈｏｒｅ）和佛波醇脂（ｐｈｏｒｂｏｌ１２ｍｙｒｉｓｔａｔｅ１３ａｃｅｔａｔｅ,ＰＭＡ）能明显提高星形胶质细胞组织因子活性的表达,而三氟吡啦嗪（ｔｒｉｆｌｕｏｐｅｒａｚｉｎｅ,ＴＦＰ）和１（５异喹啉磺胺）３甲基哌嗪［１（５ｉｓｏｑｕｉｎｏｌｉｎｙｌｓｕｌｆｏｎｙｌ）３ｍｅｔｈｙｌｐｉｐｅｒａｚｉｎｅ,Ｈ７］则降低星形胶质细胞组织因子活性的表达。凝血酶能明显增加星形胶质细胞表达组织因子活性;当凝血酶与ＴＦＰ或Ｈ７联合应用时,凝血酶的刺激作用受到明显抑制。实验表明,星形胶质细胞在基础培养条件下能表达组织因子,凝血酶能刺激它表达组织因子活性。Ｃａ２＋／钙调素和ＰＫＣ途径参与了在基础条件以及凝血酶刺激条件下星形胶质细胞组织因子活性的表达。
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| 关 键 词： | 星形胶质细胞 组织因子 蛋白激酶C 钙调素 凝血酶 |
Expression of tissue factor of astrocytes and its signal transductional pathways |
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| Zhu FM,Wen ZB,He XF,Li JC,He SL.Expression of tissue factor of astrocytes and its signal transductional pathways[J].Acta Physiologica Sinica,1999,51(3):291-296. |
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| Authors: | Zhu F M Wen Z B He X F Li J C He S L |
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| Institution: | Department of Physiology, Hunan Medical University, Changsha 410078. |
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| Abstract: | The aim of this study was to observe the expression of tissue factor(TF) of astrocytes in basic culture medium and under the condition stimulated by thrombin and to explore the relevant signal transduction pathways. The results showed that 4 bromo calcium ionophore (A 23187 ) and phorbol 12 myristate 13 acetate (PMA) enhanced significantly the TF expression of astrocytes, but the expression was decreased markedly by trifluoperazine (TFP) and 1 (5 isoquinolinyl sulfonyl) 3 methyl piperazine (H 7) in the basic medium. Thrombin increased significantly the TF expression of astrocytes, which was obviously inhibited by TFP and H 7. The results above indicate that astrocytes can express TF activity in the basic medium, which is promoted by thrombin, probably through some pathways involving Ca 2 /CaM and protein kinase C (PKC). |
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| Keywords: | astrocyte tissuse factor protein kinase C calmodulin thrombin |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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