Isatin: Identity with the Purified Endogenous Monoamine Oxidase Inhibitor Tribulin |
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Authors: | Vivette Glover J M Halket P J Watkins Angela Clow B L Goodwin M Sandier |
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Institution: | Department of Chemical Pathology, Queen Charlotte's Hospital, London, England. |
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Abstract: | Purified tribulin, an endogenous monoamine oxidase (MAO) inhibitor, has been identified by direct probe insertion mass spectrometry as the indole-2,3-dione, isatin. A gas chromatographic-mass spectrometric assay for isatin has been developed and used to measure its relatively high concentrations in unpurified human urine, and in rat heart and brain. Isatin is a known compound with a broad range of biological activity; this is the first report of its presence in the animal body. Isatin is a potent inhibitor of MAO, particularly of MAO B (IC50, 3 microM), and also binds to central benzodiazepine receptors (IC50 against clonazepam, 123 microM). |
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Keywords: | Tribulin Isatin Endogenous monoamine oxidase inhibitor Endogenous benzodiazepine receptor ligand |
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