CAPS and DHPLC Analysis of a Single Nucleotide Polymorphism in the Cytochrome b Gene Conferring Resistance to Strobilurins in Field Isolates of Blumeria graminis f. sp. hordei |
| |
| Authors: | S. Bä umler,F. G. Felsenstein, G. Schwarz |
| |
| Affiliation: | Authors' addresses: Chair of Agronomy and Plant Breeding, Department of Plant Sciences, Center of Life and Food Sciences Weihenstephan, Technical University Munich, D-85350 Freising, Germany;;EpiGene GmbH, Biotechnology in Plant Protection, D-85354 Freising, Germany (correspondence to G. Schwarz. E-mail: ) |
| |
| Abstract: | A single nucleotide polymorphism in the wheat powdery mildew (Blumeria graminis f. sp. tritici) cytochrome b gene is responsible for resistance to inhibitors of the quinol outer binding site of the cytochrome bc1 complex (QoI) fungicides. Analysis of a partial sequence of the cytochrome b gene from field isolates resistant and sensitive to QoI fungicides revealed the same point mutation in barley powdery mildew (B. graminis f. sp. hordei). Analysis of 118 and 40 barley powdery mildew isolates using a cleaved amplified polymorphic sequence assay and denaturing high performance liquid chromatography, respectively, confirmed that this single nucleotide polymorphism also confers resistance to QoI fungicides in barley powdery mildew. |
| |
| Keywords: | Blumeria graminis f. sp. hordei fungicide resistance strobilurin QoI fungicide CAPS DHPLC |
|
|
