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Expression and purification strategies for the production of single-chain antibody and T-cell receptor fragments inE. coli |
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| Authors: | Peter E. Molloy Barbara M. Graham Pauline M. Cupit Steven D. Grant Andrew J. R. Porter Charles Cunningham |
| Affiliation: | (1) Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, AB9 1AS Aberdeen, Scotland |
| Abstract: | This work describes protocols for the production of single-chain antibody and T-cell receptor fragments inE. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be denatured and refolded before the fragments attain their proper conformation. |
| Keywords: | scAb Antibody fragments Immunoglobulins scTCR T-cell receptors Metal chelate affinity chromatography Bacterial expression systems |
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