Conditional gene knockout using cre recombinase |
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Authors: | Yunzheng Le Dr Brian Sauer |
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Institution: | (1) Developmental Biology Program, Oklahoma Medical Research Foundation, 73104 Oklahoma City, OK |
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Abstract: | Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired
genetic manipulations rely on Cre’s ability to direct spatially and temporally specified excision of a predesignated DNA sequence
that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional
alleles and on reliable detection of cre gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged
Cre protein. |
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Keywords: | Cre site-specific recombination knockout FACS ES cells GFP STOP cassette fusion proteins transgenic mice loxP |
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