Differential regulation of manganese peroxidases and characterization of two variable MnP encoding genes in the white-rot fungus Physisporinus rivulosus |
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| Authors: | Terhi K Hakala Kristiina Hildén Pekka Maijala Cia Olsson Annele Hatakka |
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| Institution: | (1) Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, Biocenter 1, 00014 Helsinki, Finland;(2) Program of Developmental and Reproductive Biology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland;(3) Present address: KCL Science and Consulting, Espoo, Finland |
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| Abstract: | Manganese peroxidase (MnP) production in the white-rot basidiomycete Physisporinus rivulosus T241i was studied. Separate MnP isoforms were produced in carbon-limited liquid media supplemented with Mn2+, veratryl alcohol, or sawdust. The isoforms had different pH ranges for the oxidation of Mn2+ and 2,6-dimethoxyphenol. Although lignin degradation by white-rot fungi is often triggered by nitrogen depletion, MnPs of P. rivulosus were efficiently produced also in the presence of high-nutrient nitrogen, especially in cultures supplemented with veratryl alcohol. Two MnP encoding genes, mnpA and mnpB, were identified, and their corresponding cDNAs were characterized. Structurally, the genes showed marked dissimilarity, and the expression of the two genes implicated quantitative variation and differential regulation in response to manganese, veratryl alcohol, or sawdust. The variability in regulation and properties of the isoforms may widen the operating range for efficient lignin degradation by P. rivulosus. |
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| Keywords: | Manganese peroxidase White-rot fungi Physisporinus rivulosus Gene expression Mn2+ oxidation Lignin degradation |
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