Degradation studies on Escherichia coli capsular polysaccharides by bacteriophages |
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Authors: | Wolfgang Nimmicha |
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Affiliation: | Institut für Medizinische Mikrobiologie, Medizinische Fakultät, Universität Rostock, Schillingallee 70, D-18057 Rostock, Germany |
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Abstract: | The serologically and structurally related Escherichia coli capsular polysaccharides (K antigens) K13, K20, and K23 were found to be depolymerized by the bacteriophages ΦK13 and ΦK20 to almost similar oligomer profiles as shown by polyacrylamide gel electrophoresis. The phage-polysaccharide interactions were followed by an increase of reducing 2-keto-3-deoxyoctulosonic acid due to a phage-associated glycanase that catalyzed the hydrolytic cleavage of common β-ketopyranosidic 2-keto-3-deoxyoctulosonic acid linkages. The related E. coli K antigens K18, K22, and K100 as well as the Haemophilus influenzae type b capsular polysaccharide were degraded by bacteriophage ΦK100 with different efficacy. It is suggested that ΦK100 enzymatically cleaves ribitol-5-phosphate bonds as the only structural feature present in all the polysaccharides investigated. |
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Keywords: | Escherichia coli K-specific bacteriophage Capsular polysaccharide Depolymerase enzyme |
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