Assay for soil urease activity |
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| Authors: | P. B. May L. A. Douglas |
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| Affiliation: | (1) Soil Science Section, Faculty of Agriculture and Forestry, University of Melbourne, 3052 Parkville, Victoria, Australia |
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| Abstract: | Summary A procedure is described that allows assay of soil urease activity. The method uses a phosphate buffer (pH 8.8) and a urea substrate concentration of 0.007 M. Incubation for 4 h at 37°C is recommended and urease activity is estimated by determining the amount of ammonium produced by urea hydrolysis in soil. The method is precise, and compares favourably with other procedures. re]19750710 |
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