Quantitative gas chromatographic analysis of amino acids on a short glass capillary column |
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Authors: | Jörgen Jönsson Julius Eyem John Sjöquist |
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Affiliation: | Department of Biochemistry, Faculty of Pharmacy, The Wallenberg Laboratory, University of Uppsala, 751 22 Uppsala, Sweden |
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Abstract: | A study of the quantitative gas chromatographic analysis of protein amino acids as their N-heptafluorobutyric amino acid n-propyl esters on a glass capillary column has been made. The analysis is completed within 35 min with good separation of the common protein amino acids in a single-column run.Hydrolyzed peptides have been analyzed. The analyses were performed with a precision varying between 1 and 6% (mean relative standard deviation) depending on the number of amino acid residues in the peptide. The amount taken for analysis was 20–300 μg. The results agree with the known sequences of the peptides and with the analyses by ion-exchange chromatography except for cysteine. This amino acid can be analyzed after modification as S-methylated cysteine. |
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