Modification of serine residues during sequential degradation of proteins and peptides by the phenylisothiocyanate method |
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| Authors: | M Pavlík V Kostka |
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| Affiliation: | 1. Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic;2. Biomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic;3. 1st Department of Medicine, Faculty of Medicine in Hradec Kralove, Charles University in Prague, University Hospital, Sokolska 581, 500 05 Hradec Kralove, Czech Republic |
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| Abstract: | A novel method of serine determination during Edman degradation of proteins and peptides which is based on the reaction of serine degradation products with alkyl thiols is described. The physicochemical characteristics and the structures of the reaction products, i.e., of 5-[alkyl-thio)methyl)-3-phenyl-2-thioxo-4-imidazolinones, were determined. The procedure described permits the products of serine degradation to be obtained in a 70-80% yield. |
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