香椿试管苗的生根与移栽

通过基内诱导生根、基外诱导生根及生根苗移栽等试验,研究了香椿试管苗的生根与移栽。结果表明,基内诱导生根的主要影响因子是生长素IBA,其次是培养基中无机盐浓度,适宜的生根配方是在不含无机盐的培养基中附加1.0mg/L IAB。用NAA、IAB短时间处理无根苗基部,可使部分苗生根,但生根率偏低。试管苗移栽的适宜时期为3月至7月初,以蛭石、河沙为基质效果较好。移载成活的关键是充分炼苗及良好的基质和适宜的时期,注意防虫并保温遮荫。

ROOTING AND TRANSPLANTING OF TISSUE CULTURED SHOOTS OF TOONA SINENSIS

It was studies that the mains factor in fluencing rooting and transplanting of tissue cultured shoots of Toona sinensis . The results were summarized as follows : the main factor influencing rooting of tissue cultured shoots of Toona sinensis is IBA,and the contents of macronutrients in MS medium come second.The proper rooting medium is single IBA 1.0mg/L in MS medium without macronu-tients.The rooting rates was lowly with high concentration of NAA or IBA in a short time. The proper transplanting period is from March to early July every year.Vermiculites or sands is a good transplanting medium.The key to ensure successful transplantation is exercising plants effectively,proper transplanting medium and time.At the same time it is also important that preventing insects,shading and keeping air humidity.