A simple and fast electrophoretic method for elution of nucleic acids from gels |
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| Authors: | Younes Chouikh Michel Volovitch Pierre Yot |
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| Institution: | (1) Section de Biologie, Institut Curie, Paris, (France);(2) Institut de Recherche en Biologie Moleculaire, Universite Paris VII, Paris, (France) |
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| Abstract: | A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here. |
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