| 鸡传染性法氏囊病病毒Gt株VP5基因缺失株感染性克隆的构建 |
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| 引用本文： | 秦立廷,祁小乐,高玉龙,高宏雷,步志高,王笑梅.鸡传染性法氏囊病病毒Gt株VP5基因缺失株感染性克隆的构建[J].微生物学报,2008,48(12):1666-1670. |
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| 作者姓名： | 秦立廷 祁小乐 高玉龙 高宏雷 步志高 王笑梅 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,禽传染病研究室,哈尔滨,150001 |
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| 基金项目： | 国家“973项目”—— 国家重点基础研究发展规划项目(2005CB523202) |
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| 摘 要： | 传染性法氏囊病病毒 (IBDV)是双链，双节段RNA病毒，其基因组由A、B两个节段组成，编码结构蛋白VP1-VP4和非结构蛋白VP5。【目的】利用反向遗传操作构建拯救VP5基因缺失重组IBDV。【方法】利用体外定点突变技术，缺失IBDV Gt株VP5基因，通过多重PCR在基因组两端分别引入锤头状核酶序列(HamRz)和丁肝病毒核酶序列(HdvRz)。将带有核酶序列的IBDV基因组插入载体pCAGG的b肌动蛋白启动子下游，构建了IBDV感染性克隆pCAGGmGtA △VP5HRT，将该感染性克隆与pCAGGmGtBHRT共转染DFⅠ细胞。【结果】RT-PCR和间接免疫荧光均显示获得重组病毒，将其命名为rmGtA △VP5。IBDV VP5基因缺失感染性克隆的成功构建为从分子水平上深入研究vp5基因功能奠定了基础。
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| 关 键 词： | 传染性法氏囊病病毒 VP5 感染性克隆 重组病毒 |
| 修稿时间： | 9/2/2008 12:00:00 AM |
Cloning of infectious bursal diseasa virus(Gt strain)Lack of VP5gene |
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| Liting Qin,Xiaole Qi,Yulong Gao,Honglei Gao,Zhigao Bu,and Xiaomei Wang.Cloning of infectious bursal diseasa virus(Gt strain)Lack of VP5gene[J].Acta Microbiologica Sinica,2008,48(12):1666-1670. |
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| Authors: | Liting Qin Xiaole Qi Yulong Gao Honglei Gao Zhigao Bu and Xiaomei Wang |
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| Institution: | Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin;Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin;Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin;Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin;Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin;Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Chin |
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| Abstract: | |
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| Keywords: | infectious bursal disease virus viral protein 5 infectious clone virus rescue |
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