Alpha-tocopheryl succinate induces cytostasis and apoptosis in osteosarcoma cells: the role of E2F1 |
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Authors: | Alleva Renata Benassi Maria Serena Tomasetti Marco Gellert Nina Ponticelli Francesca Borghi Battista Picci Piero Neuzil Jiri |
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Institution: | Department of Anaesthesiology, Rizzoli Orthopaedic Institute, 40100 Bologna, Italy. renalle@libero.it |
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Abstract: | Alpha-tocopheryl succinate (alpha-TOS), a redox-inactive analog of vitamin E, induces cell cycle arrest, differentiation, and triggers apoptosis. We examined the ability of alpha-TOS to induce cytostasis and/or apoptosis in two human osteosarcoma cell lines, which carry wild-type pRb but differ in the p53 status. In the wt-p53 cells, alpha-TOS induced apoptosis, which was associated with p53 activation and enhanced E2F1 expression. Mutant p53 cells failed to undergo apoptosis when challenged with alpha-TOS. The cell growth arrest after alpha-TOS treatment was associated with a reduced expression of E2F1. Knocking down E2F1 rendered the alpha-TOS-sensitive cells rather resistant to the apoptotic stimulus inducing a marked and prolonged cell growth arrest. We conclude that alpha-TOS induces cell growth arrest or apoptosis involving E2F1. |
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Keywords: | Osteosarcoma Proliferation Apoptosis α-Tocopheryl succinate E2F1 p53 |
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