The clonogenic potential of hematopoietic stem cells and mesenchymal stromal cells in various hematologic diseases: a pilot study |
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| Authors: | Meltem Kurt Yüksel Pervin Topçuo?lu Mevlude Kurdal Osman ?lhan |
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| Institution: | 1. Department of Hematology, Ankara Oncology Research and Education Hospital;2. Department Of Hematology, Ankara University School of Medicine;3. Department of Hematology, Ankara Oncology Research and Education Hospital;4. Department of Hematology, Ankara University School of Medicine, Ankara, Turkey;1. Division of Hematology/Oncology, Department of Medicine,Rochester;2. Department of Pathology and Laboratory Medicine,Rochester;3. Department of Pharmacology and Physiology, Rochester;4. Division of Endocrinology and Metabolism, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY;1. Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY;2. Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT;3. Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY;4. Myelodysplastic Syndromes Center;5. Department of Medicine, Division of Nephrology, College of Physicians and Surgeons, Columbia University, New York, NY;6. Regional Bone Center, Helen Hayes Hospital, West Haverstraw, New York, NY;7. Amgen Inc., Seattle, WA;8. Bone and Cartilage Biology Group, Genome Engineering Technologies Group, Regeneron Pharmaceuticals Inc., Tarrytown, NY;9. Department of Medicine, Memorial Sloan Kettering Cancer Center, Weill Cornell Medical College, New York, NY;10. Department of Medicine, Division of Hematology and Oncology;11. Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY;1. Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, China;2. Department of Infectious Disease, 324 Hospital of People''s Liberation Army (PLA), Chongqing, China;1. Departments of Internal Medicine (Hematology);2. Pediatrics (Hematology-Oncology);3. Immunobiology, Yale University, New Haven, CT;4. Yale Cancer Center, Yale University, New Haven, CT;1. Department of Gastroenterology, Peking University Third Hospital, Beijing, China;2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China;3. Department of Gastroenterology, First Affiliated Hospital of SunYat-sen University, Guangzhou, China;4. Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Technology and Science, Wuhan, China;5. Department of Digestive Diseases, Changhai Hospital of Second Military Medical University, Shanghai, China |
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| Abstract: | Background aimsMesenchymal stromal cells (MSC) are the most popular cells used in regenerative medicine and biotechnology. The clonogenic potential of these cells is defined by colony-forming unit-fibroblasts (CFU-F). It is well known that there is an interaction between hematopoietic cells and stromal cells in disease formation pathogenesis. Therefore we hypothesized that there should be a quantitative and qualitative relationship between MSC colonies (CFU-F) and hematopoietic stem cell colonies (colony-forming unit-granulocyte-macrophages; CFU-GM) among patients with and without hematologic diseases.MethodsForty-two patients were included in this study. Patients were divided into three groups: group A, patients with hematologic malignancies (n = 20); group B, patients with bone marrow (BM) failure (n = 11); group C, patients without hematologic diseases (n = 11). BM aspirates were plated in different densities for CFU-F culture. The plating density was the same for CFU-GM culture.ResultsCFU-GM colonies grew in 90% of group A cells and all of group B and C cells (P = 0.0001). CFU-F colonies became visible on the ninth day of plating in group A and on the eight day in groups B and C. There was no statistically significant difference between the groups for the duration of CFU-F colony formation (P = 0.12). There were differences in the morphology of the colonies among the groups.ConclusionsThis is the first study that has compared the clonogenic potential of stromal cells and hematopoietic stem cells in the same subjects with and without hematologic diseases. No correlation was shown between the clonogenic potential of stromal cells and hematopoietic cells. |
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