Superparamagnetic iron oxide nanoparticles may affect endothelial progenitor cell migration ability and adhesion capacity |
| |
| Authors: | Jin-Xiu Yang Wei-Liang Tang Xing-Xiang Wang |
| |
| Institution: | 1. Departamento de Química Orgánica y Farmacéutica, University of Navarra, Irunlarrea, 1, E-31008 Pamplona, Spain;2. Instituto de Salud Tropical, University of Navarra, Irunlarrea, 1, E-31008 Pamplona, Spain;3. Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XR, Scotland, UK;4. Departamento de Ciencias de la Salud, Universidad Pública de Navarra, Avda. Barañáin s/n, E-31008 Pamplona, Spain;1. Department of Veterinary Medicine, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China;2. Unit for Laboratory Animal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, USA;3. College of Literature, Science, and the Arts, University of Michigan, Ann Arbor, MI 48109, USA;4. School of Information, University of Michigan, Ann Arbor, MI 48109, USA;5. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA;6. Center for Computational Medicine and Biology, University of Michigan, Ann Arbor, MI 48109, USA;7. Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA;3. From the Departments of Pharmacology and;4. Chemistry and the Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee 37232 and;5. the Department of Chemistry, Wayne State University, Detroit, Michigan 48202 |
| |
| Abstract: | Background aimsCell labeling with superparamagnetic iron oxide (SPIO) nanoparticles enables non-invasive tracking of transplanted cells. The aim of this study was to investigate whether SPIO nanoparticles have an effect on endothelial progenitor cell (EPC) functional activity and the feasibility of a protocol for labeling swine- and rat-origin EPC using SPIO nanoparticles at an optimized low dosage.MethodsEPC were isolated from the peripheral blood of swine and bone marrow of rat and characterized. After ex vivo cultivation, EPC were labeled with SPIO nanoparticles (to make a series of final concentrations, 50, 100, 200 and 400 μg/mL) or vehicle control. We also investigated the long-term effects of 200 μg/mL SPIO nanoparticles on EPC (4, 8, 12 and 16 days after labeling). The labeling efficiency was tested through Prussian blue (PB) staining and the intracellular iron uptake was also measured quantitatively and confirmed. EPC proliferation and migration were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and transwell chamber assay, respectively. An EPC adhesion assay was performed by replating the cells on fibronectin-coated dishes and then counting the adherent cells. EPC apoptosis was evaluated using an Annexin V–FITC apoptosis kit.ResultsSPIO nanoparticles impaired EPC migration and promoted EPC adhesion. EPC proliferation and apoptosis were not affected. SPIO nanoparticles could label EPC efficiently at 200 μg/mL overnight without significantly affecting EPC functional activity.ConclusionsSPIO nanoparticles impaired the EPC migration ability and promoted the EPC adhesion capacity. EPC could be labeled efficiently at an appropriate concentration (200 μg/mL) without significantly affecting their functional activity. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
