Isolation and characterization of stem cells from pancreatic islet: pluripotency,differentiation potential and ultrastructural characteristics |
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| Authors: | Erdal Karaoz Selda Ayhan Gülçin Gacar Ayça Aksoy Gökhan Duruksu Alparslan Okçu P. Çetinalp Demircan A. Eker Sariboyaci Figen Kaymaz Murat Kasap |
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| Affiliation: | 1. Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, Turkey;2. Faculty of Medicine, Department of Histology and Embryology, Hacettepe University, Turkey;1. Cell Engineering Laboratory, La Paz University Hospital Research Institute, Madrid, Spain;2. Department of Internal Medicine, La Paz University Hospital, Madrid, Spain;3. Department of Plastic and Reconstructive Surgery, Santa Cristina Hospital, and Centrocim, Madrid, Spain;4. Department of Pathology, La Paz University Hospital, Madrid, Spain;1. Department of Oncology, Hematology and Respiratory Diseases, University Hospital of Modena and Reggio Emilia, Modena, Italy;2. Department of Orthopedics, University Hospital Tübingen, Tübingen, Germany;3. Institute of Clinical and Experimental Transfusion Medicine (IKET), University Hospital Tübingen, Tübingen, Germany;4. Division of Oncology/Blood and Marrow Transplantation, The Children''s Hospital of Philadelphia and The University of Pennsylvania School of Medicine, Philadelphia, PA, USA;5. Department of Neurosurgery, Stanford University School of Medicine, Stanford University, Stanford, CA, USA;1. Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;2. Department of Pathology, No.924 (No.181) Hospital of People''s Liberation Army, Guilin, Guangxi, 541002, China;1. Department of Cardiology, Faculty of Medicine, Izmir University, Izmir, Turkey;2. Department of Histology and Embryology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey;3. Department of Stem Cells, Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Institute of Health Sciences, Kocaeli, Turkey;4. Department of Cardiovascular Surgery, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey;1. Department of Hematology, Tianjin Medical University Cancer Hospital and Institute, Tianjin, China;2. Department of Hematology, First Affiliated Hospital of General Hospital of the Chinese People''s Liberation Army, Beijing, China;3. From the Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-0065, Japan,;4. the Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-ku, Kyoto 603-8555, Japan,;5. the Noguchi Institute, 1-8-1 Kaga, Itabashi, Tokyo 173-0003, Japan,;6. the Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, 1200 Matsumoto-cho, Kasugai 487-8501, Japan, and |
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| Abstract: | Background aimsStem cells (SC) in different locations have individual characteristics. Important questions to be answered include how these specialties are generated, what the mechanism underlying their generation is, and what their biologic and clinical merits are. A basic approach to answering these questions is to make comparisons between the differences and similarities among the various SC types. They may focus on aspects of biologic marker discovery, capacity of proliferation and differentiation, along with other characteristics. The aim of this study was to characterize in detail the SC isolated from pancreatic islet (PI) and compare their properties with bone marrow (BM)-derived mesenchymal stromal cells (MSC) of the rat.MethodsImmunophenotypic characteristics, proliferation capacities, telomerase activities, pluripotent-related gene expressions, ultrastructure and the potential for multilineage differentiation of PI SC and BM MSC were studied.ResultsWe found that PI SC expressed markers of embryonic SC (Oct-4, Sox-2 and Rex-1) and had a high proliferation capacity, proven also by high telomerase activities. Surprisingly, markers belonging to differentiated cells were expressed by these cells in a constitutive manner. PI SC ultrastructure showed more developed and metabolically active cells.ConclusionsThe immunocytochemical identification of both PI SC and BM MSC was demonstrated to be typical MSC. Without stimulation of differentiation markers of adipogenic, chondrogenic, neurogenic, myogenic and osteogenic cells in these SC, the expression of those markers might explain their multilineage differentiation potential. We suggest that, by reason of the respectively high telomerase activity in PI SC, they could be better candidates than BM MSC for cell replacement therapy of type 1 diabetes. |
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