Auto-transplanted mesenchymal stromal cell fate in periodontal tissue of beagle dogs |
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| Authors: | Na Wei Ping Gong Dapeng Liao Xingmei Yang Xiaoyu Li Yurong Liu Quan Yuan Zhen Tan |
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| Institution: | 1. State Key Laboratory of Oral Disease, Sichuan University, Chengdu, PR China;2. Department of Dental Implants, West China College of Stomatology, Sichuan University, Chengdu, PR China;1. School of Dentistry and Oral Health, Centre for Medicine and Oral Health, Griffith Health Institute, Griffith University (Gold Coast Campus), 16-30 High Street, Southport, QLD 4222, Australia;2. Molecular Basis of Disease Program, Griffith Health Institute, Australia;3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renminnan Road, Chengdu, Sichuan 610041, China;4. Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD 4059, Australia;1. Faculty of Dentistry, National University of Singapore, Singapore;2. Institute of Medical Biology, Agency for Science, Technology and Research, Singapore;3. Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;4. Tissue Engineering Program, Life Sciences Institute, National University of Singapore, Singapore;1. State Key Laboratory of Oral Diseases, Department of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, PR China;2. Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China;3. Department of Stomatology, Peking Union Medical College Hospital, Beijing 100730, PR China;4. State Key Laboratory of Oral Diseases, West China School of Stomatolgy, Sichuan University, Chengdu 610041, PR China;5. Department of Stomatology, Affiliated Hospital of Chengdu University of TCM, Chengdu 610075, PR China;1. State Key Laboratory of Military Stomatology Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi''an, P.R. China;2. National Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi''an, P.R. China;3. Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi''an, P.R. China |
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| Abstract: | Background aimsMesenchymal stromal cells (MSC) possess multilineage differentiation potential and characteristics of self-renewal. It has been reported that MSC can acquire characteristics of cells in the periodontal ligament (PDL) in vitro. Moreover, the transplantation of MSC has been shown to be a promising strategy for treating periodontal defects. However, little is known about the fate of MSC in periodontal tissue in vivo. The aim of this study was to trace the paths of MSC after transplantation into periodontal tissues in vivo.MethodsMSC labeled with bromodeoxyuridine (BrdU) were transplanted into periodontal defects of beagle dogs. Six weeks after surgery, the animals were killed and decalcified specimens were prepared. Migration and differentiation of MSC were detected by single/double immunohistochemistry and a combination of immunohistochemistry and in situ hybridization.ResultsBrdU-labeled MSC were observed distributing into periodontal tissue that included alveolar bone, PDL, cementum and blood vessels and expressing surface markers typical of osteoblasts and fibroblasts.ConclusionsCumulatively, our data suggest that MSC migrate throughout periodontal tissue and differentiate into osteoblasts and fibroblasts after transplantation into periodontal defects at 6 weeks in vivo, and have the potential to regenerate periodontal tissue. |
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