Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions |
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| Authors: | Shinji Takenaka Qi Zhou Ampin Kuntiya Phisit Seesuriyachan Shuichiro Murakami Kenji Aoki |
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| Affiliation: | (1) Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokko, Kobe 657-8501, Japan;(2) Department of Biosystems Science, Division of Biosystems Chemistry Graduate School of Science and Technology, Kobe University, Rokko, Kobe 657-8501, Japan;(3) Department of Biotechnology, Faculty of Agro-industry, Chiang Mai University, Chiang Mai, 50100, Thailand |
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| Abstract: | A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3 at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+ to the NH4NO3 medium markedly promoted the utilization of NH4+ and NO3−. At 50°C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH4 15NO3 had been converted to 15N2O. These results indicate that the strain has denitrification ability under aerobic condition. |
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| Keywords: | Aerobic condition Ammonium nitrate Bacillus licheniformis Denitrification Ferrous ion Thermotolerant bacterium |
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