Magnetically empowered bone marrow cells as a micro-living motor can improve early hematopoietic reconstitution |
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| Institution: | 1. Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China;2. Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China;3. Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China;1. University College London Cancer Institute, London, UK;2. Department of Hematology, University College London Hospital, London, UK;3. Cancer Research UK & University College London Cancer Trials Center, University College London, London, UK;1. Pediatrics, University of California, San Francisco, San Francisco, California, USA;2. Crispr Therapeutics AG, Boston, Massachusetts, USA;3. ISCT Immune-Gene Therapy Committee, ISCT, Vancouver, California, USA;1. Biotherapy Center, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;2. Cancer Center, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;3. Department of Thoracic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;4. School of Life Sciences, Zhengzhou University, Zhengzhou, China;5. State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou, China |
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| Abstract: | Background aimsBone marrow-derived hematopoietic stem cell transplantation/hematopoietic progenitor cell transplantation (HSCT/HPCT) is widely used and one of the most useful treatments in clinical practice. However, the homing rate of hematopoietic stem cells/hematopoietic progenitor cells (HSCs/HPCs) by routine cell transfusion is quite low, influencing hematopoietic reconstitution after HSCT/HPCT.MethodsThe authors developed a micro-living motor (MLM) strategy to increase the number of magnetically empowered bone marrow cells (ME-BMCs) homing to the bone marrow of recipient mice.ResultsIn the in vitro study, migration and retention of ME-BMCs were greatly improved in comparison with non-magnetized bone marrow cells, and the biological characteristics of ME-BMCs were well maintained. Differentially expressed gene analysis indicated that ME-BMCs might function through gene regulation. In the in vivo study, faster hematopoietic reconstitution was observed in ME-BMC mice, which demonstrated a better survival rate and milder symptoms of acute graft-versus-host disease after transplantation of allogeneic ME-BMCs.ConclusionsThis study demonstrated that ME-BMCs serving as MLMs facilitated the homing of HSCs/HPCs and eventually contributed to earlier hematopoietic reconstitution in recipients. These data might provide useful information for other kinds of cell therapies. |
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