Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae
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| Authors: | Tohru Miyoshi-Akiyama Kayoko Hayakawa Norio Ohmagari Masahiro Shimojima Teruo Kirikae |
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| Affiliation: | 1. Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan.; 2. Disease Control and Prevention Center, National Center for Global Health and Medicine, Toyama, Shinjuku-ku, Tokyo, Japan.; 3. BML Inc., Matoba, Kawagoe, Saitama, Japan.; The University of Hong Kong, China, |
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| Abstract: | Enterobacter cloacae is an important emerging pathogen, which sometime causes respiratory infection, surgical site infection, urinary infection, sepsis, and outbreaks at neonatal units. We have developed a multilocus sequence typing (MLST) scheme utilizing seven housekeeping genes and evaluated the performance in 101 clinical isolates. The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study. |
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