Cell monitoring with optical coherence tomography |
| |
| Affiliation: | 1. ChromoLogic LLC, Monrovia, California, USA;2. Protein Expression Center, California Institute of Technology, Pasadena, California, USA;1. Pediatrics, University of California, San Francisco, San Francisco, California, USA;2. Crispr Therapeutics AG, Boston, Massachusetts, USA;3. ISCT Immune-Gene Therapy Committee, ISCT, Vancouver, California, USA;1. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;2. Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan, China;3. Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;1. Kantonsspital Frauenfeld, Frauenfeld, Switzerland;2. Department of Plastic Surgery and Hand Surgery, University Hospital Zurich, Zurich, Switzerland;1. Chemistry, Manufacturing and Control Regulatory Affairs, Regulatory Affairs, Astellas Pharma Inc, Tokyo, Japan;2. Japan Tissue Engineering Co, Ltd, Aichi, Japan;3. Acepodia Inc, Taipei, Taiwan;4. Strategic Center for Regenerative Medicine, Inha University College of Medicine, Incheon, South Korea;5. China Medicinal Biotech Association, Beijing, China;6. Stempeutics Research Pvt, Ltd, Bangalore, India;7. EMO Biomedicine, Taipei, Taiwan;8. CEFO Co, Ltd, Seoul, South Korea;9. Baylx, Inc, Beijing, China;10. Shenzhen Easeng Medical Science Co., LTD, Shenzhen, China;11. Astellas Pharma Inc, Tsukuba, Japan;1. School of Biological Sciences, The University of Auckland, Auckland, New Zealand;2. Department of Surgery, Faculty of Medicine and Health Sciences, The University of Auckland, Auckland, New Zealand;3. Counties Manukau District Health Board, Auckland, New Zealand;4. Maurice Wilkins Centre, The University of Auckland, Auckland, New Zealand;1. CReATe Fertility Center, Toronto, Ontario, Canada;2. Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada;3. Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada;4. Department of Physiology, University of Toronto, Toronto, Ontario, Canada |
| |
| Abstract: | Background aimsWe evaluated a commercially available instrument, OCTiCell (chromologic.com/octicell), for monitoring cell growth in suspended agitated bioreactors based on optical coherence tomography. OCTiCell is an in-line, completely non-invasive instrument that can operate on any suspended-cell bioreactor with a window or transparent wall. In traditional optical coherence tomography, the imaging beam is rastered over the sample to form a three-dimensional image. OCTiCell, instead uses a fixed imaging beam and takes advantage of the motion of the media to move the cells across the interrogating optical beam.ResultsWe found strong correlations between the non-invasive, non-contact, reagent-free OCTiCell measurements of cell concentration and viability and those obtained from the automated cell counter, and the XTT viability assay, which is a colorimetric assay for quantifying metabolic activity.ConclusionsThis novel cell monitoring method can adapt to different bioreactor form factors and could reduce the labor cost and contamination risks associated with cell growth monitoring. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
