Overexpression of initiator methionine tRNA leads to global reprogramming of tRNA expression and increased proliferation in human epithelial cells |
| |
Authors: | Mariana Pavon-Eternod Suzana Gomes Marsha R Rosner Tao Pan |
| |
Institution: | 1.Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA;2.Ben May Department of Cancer Research, University of Chicago, Chicago, Illinois 60637, USA |
| |
Abstract: | Transfer RNAs (tRNAs) are typically considered housekeeping products with little regulatory function. However, several studies over the past 10 years have linked tRNA misregulation to cancer. We have previously reported that tRNA levels are significantly elevated in breast cancer and multiple myeloma cells. To further investigate the cellular and physiological effects of tRNA overexpression, we overexpressed tRNAiMet in two human breast epithelial cell lines. We then determined tRNA abundance changes and performed phenotypic characterization. Overexpression of tRNAiMet significantly altered the global tRNA expression profile and resulted in increased cell metabolic activity and cell proliferation. Our results extend the relevance of tRNA overexpression in human cells and underscore the complexity of cellular regulation of tRNA expression. |
| |
Keywords: | initiator methionine tRNA tRNA tRNA microarrays |
|
|