New pollen-specific receptor kinases identified in tomato,maize and Arabidopsis: the tomato kinases show overlapping but distinct localization patterns on pollen tubes |
| |
Authors: | Kim Hyun Uk Cotter Robyn Johnson Sheila Senda Mineo Dodds Peter Kulikauskas Rima Tang Weihua Ezcurra Ines Herzmark Paul McCormick Sheila |
| |
Affiliation: | (1) Plant Gene Expression Center, USDA/ARS-UC-Berkeley, 800 Buchanan St., Albany, CA 94710, USA;(2) Department of Botany, UC-Riverside, Riverside, CA 92521, USA;(3) Gene Research Center, Hirosaki University, Hirosaki, 036, Japan;(4) CSIRO-Plant Industry, GPO Box 1600, Canberra, 2601, Australia;(5) Developmental Biology, Duke University, Durham, NC 27708, USA;(6) Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, 751 23 Uppsala, Sweden;(7) Department of Cellular and Molecular Pharmacology, UCSF, 450 Parnassus St., San Francisco, CA 94305, USA |
| |
Abstract: | We previously characterized LePRK1 and LePRK2, pollen-specific receptor kinases from tomato (Muschietti et al., 1998). Here we identify a similar receptor kinase from maize, ZmPRK1, that is also specifically expressed late in pollen development, and a third pollen receptor kinase from tomato, LePRK3. LePRK3 is less similar to LePRK1 and LePRK2 than either is to each other. We used immunolocalization to show that all three LePRKs localize to the pollen tube wall, in partially overlapping but distinct patterns. We used RT-PCR and degenerate primers to clone homologues of the tomato kinases from other Solanaceae. We deduced features diagnostic of pollen receptor kinases and used these criteria to identify family members in the Arabidopsis database. RT-PCR confirmed pollen expression for five of these Arabidopsis candidates; two of these are clearly homologues of LePRK3. Our results reveal the existence of a distinct pollen-specific receptor kinase gene family whose members are likely to be involved in perceiving extracellular cues during pollen tube growth. |
| |
Keywords: | gene family glycosylation immunolocalization patterns ligand pollen tube growth receptor-like kinase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|