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A Simple and Rapid Method for the Purification of Ovine Pineal Arylalkylamine N-Acetyltransferase |
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| Authors: | M A A Namboodiri M J Brownstein Pierre Voisin Joan L Weller David C Klein |
| Institution: | Section on Neuroendocrinology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development;Laboratory of Cell Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, U.S.A. |
| Abstract: | A two-step chromatographic procedure has been developed for the purification of ovine pineal arylalkylamine N-acetyltransferase (EC 2.3.1.87), based on the principles of disulfide exchange and anion exchange. The enzyme from 20 ovine pineal glands can be purified about 500-fold in a day; recovery is about 5%. Polyacrylamide gel electrophoretic analysis of the final preparation shows four major bands; one appears to be arylalkylamine N-acetyltransferase. |
| Keywords: | Cystamine Disulfide exchange Fast protein liquid chromatography Anion exchange |