Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates |
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Authors: | Drzewiecka Dominika Toukach Philip V Arbatsky Nikolay P Zych Krystyna Shashkov Alexander S Knirel Yuriy A Sidorczyk Zygmunt |
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Institution: | Department of General Microbiology, Institute of Microbiology and Immunology, University of ?odz, 90-237 ?odz, Poland. |
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Abstract: | The O-specific polysaccharide of the lipopolysaccharide of Proteus penneri strain 103 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H,(13)C HMQC, 1H, 31P HMQC, and HMBC experiments. It was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of Gram-positive bacteria. The following structure of the polysaccharide was established:where Etn and Rib-ol are ethanolamine and ribitol, respectively. This structure is unique among the known structures of Proteus O-antigens and, therefore, we propose classification of the strain studied into a new Proteus serogroup, O73. The molecular basis for cross-reactivity between O-antiserum against P. penneri 103 and O-antigens of P. mirabilis O33 and D52 is discussed. |
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Keywords: | Proteus penneri O-antigen O-specific polysaccharide Lipopolysaccharide Ribitol phosphate Ethanolamine phosphate 1H 13C 31P NMR |
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