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A 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus enhances acyl exchange between acyl-CoA and phosphatidylcholine
Authors:Olga P. Yurchenko,Cory L. Nykiforuk,Maurice M. Moloney,Ulf Stå  hl,Antoni Bana&#  ,Sten Stymne, Randall J. Weselake
Affiliation:Agricultural Lipid Biotechnology Program, Department of Agricultural, Food and Nutritional Science, 4-10 Agriculture/Forestry Centre, University of Alberta, Edmonton, AB, Canada, T6G 2P5;
SemBioSys Genetics Inc., #110 2985 –23 Avenue N.E., Calgary, AB, Canada, T1Y 7L3;
Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, P.O. Box 101, S 230 53 Alnarp, Sweden;
Intercollegiate Faculty of Biotechnology, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland
Abstract:The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus was over-expressed in developing seeds of Arabidopsis thaliana . Biochemical analysis of T2 and T3 A. thaliana seeds revealed a significant increase in polyunsaturated fatty acids (FAs) (18:2 cis Δ9,12 and 18:3 cis Δ9,12,15) at the expense of very long monounsaturated FA (20:1 cis Δ11) and saturated FAs. In vitro assays demonstrated that recombinant B. napus ACBP (rBnACBP) strongly increases the formation of phosphatidylcholine (PC) in the absence of added lysophosphatidylcholine in microsomes from ΔYOR175c yeast expressing A. thaliana lysophosphatidylcholine acyltransferase ( AthLPCAT ) cDNA or in microsomes from microspore-derived cell suspension cultures of B. napus L. cv. Jet Neuf. rBnACBP or bovine serum albumin (BSA) were also shown to be crucial for AthLPCAT to catalyse the transfer of acyl group from PC into acyl-CoA in vitro . These data suggest that the cytosolic 10-kDa ACBP has an effect on the equilibrium between metabolically active acyl pools (acyl-CoA and phospholipid pools) involved in FA modifications and triacylglycerol bioassembly in plants. Over-expression of ACBP during seed development may represent a useful biotechnological approach for altering the FA composition of seed oil.
Keywords:acyl-CoA-binding protein    Arabidopsis    lysophosphatidylcholine acyltransferase    phosphatidylcholine    polyunsaturated fatty acid    triacylglycerol
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