Taurine prevents intracellular calcium overload during calcium paradox of cultured cardiomyocytes |
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Authors: | K. Takahashi Ph. D. S. W. Schaffer J. Azuma |
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Affiliation: | (1) Department of Clinical Evaluation of Medicines and Therapeutics, Faculty of Pharmaceutical Sciences, Osaka University, 565 Suita, Osaka, Japan;(2) Department of Pharmacology, University of South Alabama School of Medicine, Mobile, Alabama, USA |
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Abstract: | Summary The effect of taurine on the cellular distribution of [Ca2+]i, during the calcium paradox was examined by digital imaging of a single fura-2-loaded cell. Cardiomyocytes superfused with control medium containing 2mM Ca2+ exhibited typical transients associated with spontaneous beating. When the cells were exposed to Ca2+-free buffer, immediate cessation of both spontaneous contractions and calcium transients was observed as [Ca2+]; rapidly fell to a level of 3–6 × 10–8M. Subsequent restoration of medium calcium increased [Ca2+]i to level 4–7 times normal. Large increases in [Ca2+]i were observed in most cells and were associated with the development of contracture and bleb formation.Taurine pretreatment (20mM) caused no significant effect on [Ca2+]i during Ca2+ depletion. However, it inhibited excessive accumulation of [Ca2+]i during the Ca2+ repletion. Moreover, taurine treated cells recovered their Ca2+-transients and beating pattern earlier than non-treated cells. Finally morphological abnormalities commonly associated with calcium overload were attenuated by taurine treatment. |
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Keywords: | Amino acids Taurine Calcium paradox Cultured myocardial cell Intracellular free calcium Beating |
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