2-Amino-7-deazaadenine forms stable base pairs with cytosine and thymine. |
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| Authors: | Akimitsu Okamoto Kazuo Tanaka Isao Saito |
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| Affiliation: | Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and CREST, Japan. |
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| Abstract: | 2-Amino-7-deazaadenine ((AD)A) was incorporated into oligodeoxynucleotides (ODN) and their base-pairing properties with natural nucleobases were investigated. In melting temperature (T(m)) experiments, the duplex containing an (AD)A/C base pair showed a high stability comparable to that containing (AD)A/T base pair. Destabilization of the duplex usually observed for existing degenerate bases was not observed. However, the incorporation efficiency of dCTP was only 1.8% for TTP in single-nucleotide insertion reactions using polymerase. |
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