Cloning and expression of the isoflavone synthase gene (IFS-Tp) from Trifolium pratense |
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Authors: | Kim Bong Gyu Kim Song-Young Song Hee Su Lee Chan Hur Hor-Gil Kim Su-Il Ahn Joong-Hoon |
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Institution: | Department of Forest and Environmental Science, Bio/Molecular Informatics Center, KonKuk University, Seoul 143-701, Korea. |
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Abstract: | Isoflavones are secondary metabolites found mainly in leguminous plants. Their synthesis from flavanones is catalyzed by isoflavone synthase (IFS). We have cloned a isoflavone synthase gene (IFS-Tp) from Trifolium pratense that encodes a predicted 525 amino acids protein, molecular weight 59 kDa, with strong homology to IFS's from other legumes. IFS-Tp was expressed in all the tissues examined, and addition of glutathione and UV irradiation enhanced its expression. Microsomes from yeast transformed with IFS-Tp were able to convert naringenin to genistein, indicating that IFS-Tp has isoflavone synthase activity. |
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