首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Serological and complementation studies in four C57BL/6H-2 mutants
Authors:Ian F C McKenzie  Gillian M Morgan  Roger W Melvold  Henry I Kohn
Institution:(1) The Department of Medicine, Austin Hospital, 3084 Heidelberg, Australia;(2) Shields Warren Radiation Laboratories, Harvard Medical School, Boston, Massachusetts
Abstract:C57BL/6 (H-2 b ) mice, and four mutants (B6.C-H-2 ba , B6-H-2 bg1 , B6-H-2 bg2 , B6-H-2 bh ) derived from this strain after separate mutations had occurred at the same locus within theH-2 complex, were analyzed to determine whether the mutations had led to anyH-2 (or Ia) difference which could be detected serologically. The strains were typed directly with antisera specific for H-2K and H-2D public and private specificities and for the Ia specificities; quantitative absorption studies were also performed for the relevant H-2Kb, H-2Dd and Iab specificities. In no case was any quantitative or qualitative difference detected serologically between any of the strains. In addition, by using a variety of techniques to produce and assay for antibody, we failed to produce any antisera between the parental strains and the four mutants. TheH-2 mutations therefore appear to give rise to a type of antigenic specificity which is recognized byT cells and which generateT, but notB cell responses; nor are they recognized by H-2 or Ia alloantisera. The location of the mutating locus within theH-2 complex was shown by the complementation method to be within theK orIA region and not in theIB region, since crosses of the mutant strains with B10.A(4R) or D2.GD failed to complement for a subsequent C57BL/6 skin graft.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号