Growing colorectal tumors: minimizing microbial and stromal competition and assessing in vitro selection pressures |
| |
Authors: | Timothy M Farrell Olive S Pettengill Daniel S Longnecker Charles C Cate Kenneth H Cohn |
| |
Institution: | (1) Departments of Surgery and Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, U.S.A.;(2) Department of Surgery, University of North Carolina, CB 7210 Chapel Hill, NC 27599-7210, U.S.A. |
| |
Abstract: | Development of primary colorectal cancer cell lines ishampered by contamination from regional microbes, overgrowthof stromal cells, and purported genetic drift from selectionpressures in vitro. We initiated 32 primaryadenocarcinomas, 3 recurrences and 6 distant metastases incell culture. Twelve cell lines from eleven tumors weregenerated (26.8%) overall. Nine of 32 primary tumorsyielded 10 cell lines, 5 were lost to contamination, 13 wereoverwhelmed by stromal cells, and 5 demonstrated no growth.Addition of isobutyl methyl xanthine (IBMX) to culturelimited fibroblastoid growth. There was no associationbetween tumor location (p = 0.535, mid-P), degree ofdifferentiation (p = 0.850, mid-P) or clinicopathologic stage(p = 0.400, mid-P), and the ability of cells to becomeestablished in culture. The majority of cell lines hadsimilar nuclear DNA content and expression of cell-surfaceantigens compared with their parent tumors. Microbialcontamination and stromal cell overgrowth present thegreatest obstacle to capturing a representative bank ofcolon tumors in vitro. |
| |
Keywords: | cell culture colorectal cancer microbial contamination stroma |
本文献已被 SpringerLink 等数据库收录! |
|