Immunogenetic analysis ofH-2 mutations |
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Authors: | James Forman Jan Klein |
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Institution: | (1) Department of Microbiology, The University of Texas Southwestern Medical School, 75235 Dallas, Texas |
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Abstract: | Spleen cells carrying theH-2K
b
allele and sensitized against TNP-modified stimulator cells in vitro displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in theH-2K
b
allele (haplotypesH-2
ba
,H-2
bd
, andH-2
bf
). Similar crossreactivity in TNP-CML was observed in the reciprocal direction. Spleen cells carrying theH-2K
k
allele and sensitized against TNP-modified stimulators displayed a cytotoxic effect against TNP-modified target cells carrying a mutation in theH-2K
k
allele (haplotypeH-2
ka
) and vice versa. The effector cells in these assays were sensitive to anti-T cell serum in the presence of complement, and supernatants from immune cultures did not induce nonimmune cells to display a cytotoxic effect. Titration of effector cells from mutant and wild-type strains of theH-2
b
haplotype indicated no detectable quantitative differences in their activities. These data demonstrate that crossreactivity in TNP-CML occurs in closely related allogeneic strains that have recently undergone mutation in theH-2 complex. |
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Keywords: | |
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