Curvature of mouse satellite DNA and condensation of heterochromatin |
| |
| Authors: | M Z Radic K Lundgren B A Hamkalo |
| |
| Affiliation: | 1. Institute of Biocomputation and Physics of Complex Systems (BIFI), Joint Units GBsC-CSIC-BIFI and ICVV-CSIC-BIFI, Universidad de Zaragoza, Zaragoza 50018, Spain;2. Instituto de Investigación Sanitaria Aragón (IIS Aragón), 50009 Zaragoza, Spain;3. Josep Carreras Leukaemia Research Institute (IJC), Badalona, 08916 Barcelona, Spain;4. Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 28029 Madrid, Spain;5. Institucio Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain;6. Physiological Sciences Department, School of Medicine and Health Sciences, University of Barcelona (UB), l''Hospitalet de Llobregat, 08907 Barcelona, Spain;7. Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), 28029 Madrid, Spain;8. Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, 50009 Zaragoza, Spain;1. Department of Neurology, Nanfang Hospital, the Southern Medical University, Guangzhou, PR China;2. Department of Neurology, Zhujiang Hospital, the Southern Medical University, Guangzhou, Guangdong, PR China;3. Department of Neurology, the First People''s Hospital of Chenzhou, Chenzhou, Hunan, PR China |
| |
| Abstract: | Cloned, sequenced mouse satellite DNA exhibits properties characteristic of molecules that possess a stable curvature. Circularly permuted fragments containing the region predicted to bend were used to map the curvature relative to DNA sequence. The altered mobility of these fragments in polyacrylamide gels is reversed when gels are run in the presence of distamycin A, a drug that binds preferentially to AT-rich DNA. Treatment of living mouse cells with this drug dramatically reduces the condensation of centromeric heterochromatin, the exclusive location of satellite sequences. In situ hybridization of satellite probes to extended chromosomes at the electron microscope level shows that satellite does not comprise a single block but is distributed throughout the centromere region. Based on these experiments, we hypothesize that the structure of mouse satellite DNA is an important feature of centromeric heterochromatin condensation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
