GABA from putrescine is bound in macromolecular form in keratinocytes |
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Authors: | Zoe N Canellakis Leonard M Milstone Leslie L Marsh Paul R Young Philip K Bondy |
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Institution: | 1. West Haven Veterans Administration Medical Center, West Haven CT 06516, USA;1. Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510, USA;5. Department of Dermatology, Yale University School of Medicine, New Haven, CT 06510, USA |
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Abstract: | We report for the first time that the neurotransmitter γ-aminobutyric acid (GABA) exists in macromolecular form in keratinocytes. GABA derived from putrescine (Pu) has been identified as a component of acid-precipitable material of cultured human keratinocytes. Confluent, stratified cultures of human foreskin keratinocytes exposed to 3H]-Pu for 4 hours took up about 14% of the radioactivity from the medium and 1% of the total cell-associated radioactivity was precipitable by trichloroacetic acid (TCA). Both attached and shed cells were examined by HPLC for Pu and its radioactive metabolites in TCA-insoluble and TCA-soluble fractions. GABA accounted for the major portion (54%) of the radioactivity derived from Pu in the TCA-precipitable material of attached keratinocytes. Pu and spermidine represented lesser amounts, 35% and 9% respectively, of the total TCA-precipitable radioactivity. In addition, a large portion of acid soluble radioactivity derived from Pu (63%) was GABA, whereas Pu and spermidine represented 29% and 6% respectively of the total TCA-soluble radioactivity. The exact origin of GABA in acid-precipitable material, as well as its form of attachment, is currently under investigation. |
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