Genetic analysis of Karnal bunt (Neovossia indica) resistance in wheat |
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Authors: | M Kumar O P Luthra V Chawla N R Yadav R Kumar A Khar |
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Institution: | (1) Department of Genetics, CCS Haryana Agricultural University, 125 004 Hisar, India;(2) Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, 125 004 Hisar, India;(3) Department of Botany and Plant Physiology, CCS Haryana Agricultural University, 125 004 Hisar, India;(4) Department of Vegetable Crops, CCS Haryana Agricultural University, 125 004 Hisar, India |
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Abstract: | Embryos excised from seeds of six generations (P1, P2, F1, BC1, BC2 and F2) of a cross WH 283 x WH 533 were cultured on modified MS medium already inoculated with secondary sporidia ofNeovossia indica. Significant variations for callusing response (CR) (54.55–75.55%) were observed among generations but the presence or absence
ofN. indicia did not affect callusing response. A clear inhibition zone (IZ) was formed around each embryo showing callusing. The diameter
of IZ varied significantly among generations and was maximum in the resistant genotype, WH 283 (3.60 cm). Fresh weight and
dry weight of calli, initiated from embryo cultured and inoculated withN. indica, varied significantly among generations. Coefficient of infection as well as percentage of infection reflected the overdominance
of susceptibility. Generation mean analysis showed that the three parameter model was adequate for diameter of IZ only. Six-parameter
model showed that additive (in presence ofN. indica), additive and additive x dominance (in absence ofN. indica) effects were also significant. Complementary type of epistasis for fresh weight of calli and dominance, and dominance x dominance
effects for dry weight of calli were observed in the presence ofN. indica. Magnitude of additive effects was higher for diameter of IZ in three parameter model. Therefore, selection might assist
in improving this trait and thus indirectly help in attaining the resistance towardsN. indica. |
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Keywords: | Callusing response coefficient of infection fresh and dry weight of calli Neovossia indica |
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