Modulation of somatic embryogenesis in hypocotyl-derived cultures of geranium (Pelargonium X hortorum bailey) CV ringo rose by a bacterium |
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Authors: | Christena Visser-Tenyenhuis B. N. S. Murthy Joseph Odumeru Praveen K. Saxena |
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Affiliation: | (1) Department of Horticultural Science, University of Guelph, N1G 2W1 Guelph, Ontario, Canada;(2) Ontario Ministry of Agriculture and Food, N1H 8J7 Guelph, Ontario, Canada |
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Abstract: | Summary The Ringo Rose cultivar of zonal geranium (Pelargonium x hortorum Bailey) has been shown to be morphogenetically unresponsive. Attempts to improve somatic embryogenesis using various seed stress treatments before germination proved ineffective. However, bacterial contamination of one of the seed-stress treatments led to infected explants that had a significant increase in frequency of high-quality somatic embryos. The co-cultivation of explants with the isolated bacterium (tentatively identified asBacillus sp.) was found to be repeatable, and potentially represents a novel way to improve morphogenesis in geranium and possibly other species. |
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Keywords: | geranium Pelargonium somatic embryogenesis bacterium co-cultivation |
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