Proteome analysis of an ectomycorrhizal fungus Boletus edulis under salt shock |
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Authors: | Yu Liang Hui Chen Mingjuan Tang Shihua Shen |
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Affiliation: | aKey laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P. R. China |
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Abstract: | Soil salinization has become a severe global problem and salinity is one of the most severe abiotic stresses inhibiting growth and survival of mycorrhizal fungi and their host plants. Salinity tolerance of ectomycorrhizal fungi and survival of ectomycorrhizal inocula is essential to reforestation and ecosystem restoration in saline areas. Proteomic changes of an ectomycorrhizal fungus, Boletus edulis, when exposed to salt stress conditions (4 % NaCl, w/v) were determined using two-dimensional electrophoresis (2DE) and mass spectrometry (MS) techniques. Twenty-two protein spots, 14 upregulated and 8 downregulated, were found changed under salt stress conditions. Sixteen changed protein spots were identified by nanospray ESI Q-TOF MS/MS and liquid chromatography MS/MS. These proteins were involved in biosynthesis of methionine and S-adenosylmethionine, glycolysis, DNA repair, cell cycle control, and general stress tolerance, and their possible functions in salinity adaptation ofBoletus edulis were discussed. |
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Keywords: | Edible mushrooms ESI Q-TOF MS/MS LC MS/MS Mycorrhizas Proteins Saline soils Two-dimensional electrophoresis |
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