Carboxylase genes of Sulfolobus metallicus |
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Authors: | N P Burton T D Williams P R Norris |
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Institution: | (1) Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK e-mail: p.r.norris@warwick.ac.uk, Tel.: +44-1203-523733, Fax: +44-1203-523701, |
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Abstract: | Carbon dioxide limitation of Sulfolobus metallicus resulted in increased cellular concentrations of polypeptides that were predicted to be biotin carboxylase and biotin carboxyl-carrier-protein
components of a protein complex. These polypeptides were coeluted from a native polyacrylamide gel and were estimated at 19
and 59 kDa after separation by denaturing gel electrophoresis. Their encoding genes were identified, sequenced and shown to
code for polypeptides of 18,580 and 58,235 Da with similarities to biotin carboxyl carrier proteins and biotin carboxylases,
respectively. The genes overlapped at the second of two stop codons that terminated the carboxylase gene. A third gene occurred
on the opposite strand, 293 bp upstream of the biotin carboxylase gene. Its deduced amino acid sequence was similar to those
of carboxyl transferase subunits of carboxylase enzymes, in particular to those of the propionyl-CoA carboxylases. It is proposed
that the three described genes could encode the key enzyme complex responsible for carbon dioxide fixation during autotrophic
growth of the thermoacidophilic archaea.
Received: 24 February 1999 / Accepted: 30 July 1999 |
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Keywords: | Sulfolobus metallicus Autotrophy Carboxylases |
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