Interaction of filipin with junctional membrane at different stages of the junction's life history |
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Authors: | M A Risinger W J Larsen |
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Institution: | 1. Department of Anatomy, University of Iowa, Iowa City, Iowa 52242 USA;2. Department of Anatomy and Cell Biology, University of Cincinnati, Cincinnati, Ohio 45267 USA |
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Abstract: | The relationship of filipin-sterol complexes to tight and gap junctions during their formation, maturation, internalization, and degradation was studied in separate cell lines. Filipin-sterol complexes tended to be excluded from mature junctions in tight junction forming COLO 316 cells and gap junction forming SW-13 cells. Once internalized, unlabeled junctional membrane appeared to fuse with heavily labeled vesicles, presumably lysosomes. Although the absence of filipin-sterol complexes from junctional membrane does not necessarily reflect the absolute sterol content of this membrane, the fact that filipin-sterol complexes are largely excluded from these areas indicates that this membrane is different from surrounding membrane. The absence of filipin-sterol complexes also permits the visualization of 'mixing' of this specialized unlabeled membrane domain with other filipin labeled membrane systems. |
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Keywords: | To whom correspondence should be addressed |
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