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Micropropagation of juvenile and adult Sorbus domestica L.
Authors:Isabel Arrillaga  Toni Marzo  Juan Segura
Institution:(1) Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Avda, Blasco Ibañez 13, 46010 Valencia, Spain
Abstract:Successful propagation of seedlings and mature trees of Sorbus domestica L. has been achieved by in vitro methods. Multiple shoot formation was obtained by placing shoot apices or nodal segments on a modified Schenck and Hildebrandt medium containing benzyladenine. Regenerated shoots were excised and induced to root on media with auxin. In the best treatments 75–85% of shoots from juvenile material rooted. Rooting capacity of shoots from mature explants was lower (30%) and was not improved by dipping the base of shoots in concentration solutions of indolebutyric or naphthaleneacetic acids. Plantlets were ultimately established in soil.Abbreviations BA benzyladenine - IAA indoleacetic acid - IBA indolebutyric acid - NAA naphthaleneacetic acid
Keywords:rooting  shoot proliferation  tissue culture
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