Micropropagation of juvenile and adult Sorbus domestica L. |
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Authors: | Isabel Arrillaga Toni Marzo Juan Segura |
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Institution: | (1) Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, Avda, Blasco Ibañez 13, 46010 Valencia, Spain |
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Abstract: | Successful propagation of seedlings and mature trees of Sorbus domestica L. has been achieved by in vitro methods. Multiple shoot formation was obtained by placing shoot apices or nodal segments on a modified Schenck and Hildebrandt medium containing benzyladenine. Regenerated shoots were excised and induced to root on media with auxin. In the best treatments 75–85% of shoots from juvenile material rooted. Rooting capacity of shoots from mature explants was lower (30%) and was not improved by dipping the base of shoots in concentration solutions of indolebutyric or naphthaleneacetic acids. Plantlets were ultimately established in soil.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- NAA
naphthaleneacetic acid |
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Keywords: | rooting shoot proliferation tissue culture |
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