Effects of anisosmotic buffers on K+ transport in isolated chicken enterocytes |
| |
Authors: | M C Montero A Ilundáin |
| |
Affiliation: | Departamento Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, Spain. |
| |
Abstract: | Cells isolated by hyaluronidase incubation from chicken small intestine were used to study the effects of anisosmotic buffers on K+ transport. Hypo-osmolarity (200 mosmol.l-1) reduced both the ouabain-sensitive and the ouabain-resistant, but bumetanide-sensitive, net K+ influx and increased the K+ efflux. The hypo-osmolarity induced K+ efflux was prevented by quinine and unaffected by bumetanide. These results suggest that Ca2+-activated K+ channels may be involved in regulatory volume decrease in chicken enterocytes. Hyperosmotic conditions (400 mosmol.l-1) increased the portion of net K+ influx mediated by the Na+/K+-ATPase and that mediated by the bumetanide-sensitive K+ transport system, and decreased the K+ efflux. |
| |
Keywords: | |
|
|