Changes in beta-tubulin isoforms and their RNA level in synchronized tobacco cells. |
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Authors: | M Nishimura C Tanigaki S Okamura |
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Affiliation: | Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan. |
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Abstract: | Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a cloned carrot beta-tubulin cDNA probe, a single band of about 1.6 kb was detected throughout the cell cycle. Dot blot hybridization showed that beta-tubulin mRNA existed in all stages in the cell cycle at a relatively constant level, though it accumulated slightly more than average at M phase and decreased during G1 phase. |
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