An active cytochrome c oxidase depleted of subunit III prepared by covalent chromatography on yeast cytochrome c |
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| Authors: | K Bill A Azzi |
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| Affiliation: | Medizinisch-chemisches Institut der Universität Bern Bühlstrasse 28, CH-3012 Bern, Switzerland |
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| Abstract: | A covalent chromatography technique is described for the preparation of an active cytochrome c oxidase from bovine heart devoid of subunit III. Yeast cytochrome c is immobilized on a Sepharose 4B gel, its cysteine 107 activated and reacted with the oxidase. Elution with Triton X-100 releases an oxidase devoid of subunit III, which is recovered after elution with β-mercaptoethanol. |
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